mouse cytokine antibody array kit panel a  (R&D Systems)

Journal: Nature Aging

Article Title: Preexisting senescent fibroblasts in the aged bladder create a tumor-permissive niche through CXCL12 secretion

doi: 10.1038/s43587-024-00704-1

Figure Lengend Snippet: a , Relative protein amounts of CXCL12 in transplanted bladder cancer from young (7–12-week-old) female p16-Cre ERT2 or p16-Cre ERT2 -DTR mice using cytokine array. (p16-Cre ERT2 , n = 3; p16-Cre ERT2 -DTR, n = 4). b , Representative IHC images of bladder with MB49 cells transplanted into young (7–12-week-old) female wild-type mice with vehicle (left top) or ABT-263 (left bottom) using an anti-MKI67 antibody. The percentage of MKI67 + cells is shown in the right panel. Scale bar, 100 μm. (n = 6 for each group). c , Representative IHC images of the bladders with MB49 cell transplantation in young (7–12-week-old) female p16-Cre ERT2 (top left) or p16-Cre ERT2 -DTR (bottom left) mice using an anti-CD105 antibody. The percentage of CD105 + area was shown in the right panel. Scale bar, 100 μm. (n = 6 for each group). d , Immunoblotting images using the indicated antibodies on the indicated cultured cell lines with or without treatment with CXCL12 recombinant protein. e , qPCR of Cxcr4 and Cxcr7 on the indicated cell lines. (n = 3 for each group). f , Experimental design of transplantation of the indicated cell lines and AMD3100 treatment using young (7–12-week-old) female wild-type mice. g , Immunoblotting images using lysates of bladder tumor originated from transplanted parental MB49 cells in young (7–12-week-old) female wild-type mice. Treatment with vehicle or AMD3100 was performed on the design of f . h , Experimental design of bladder allografts with the double treatment of tamoxifen+DT and AMD3100 using young (7–12-week-old) female p16-Cre ERT2 or p16-Cre ERT2 -DTR mice. i , Bladder weights on the design of h . (p16-Cre ERT2 , n = 6; p16-Cre ERT2 -DTR, n = 5). Unpaired t-test was performed for a–c and i . One-way ANOVA with Games-Howell’s test was performed for e . Data in a–c and e are presented as mean values ± SEM. For all box plots, notch of box blots indicates median value. Lower and upper bounds correspond to 25th and 75th percentiles, respectively. Cross mark indicates mean value. Whiskers extend to maximum or minimum value, with points outside this range drawn individually. All the t-test were two-side.

Article Snippet: Using the mouse cytokine antibody array kit panel A (#ARY006, R&D Systems), we evaluated cytokines in transplanted bladder cancer.

Techniques: Transplantation Assay, Western Blot, Cell Culture, Recombinant

Journal: Pain

Article Title: Morphine-induced osteolysis and hypersensitivity is mediated through toll-like receptor-4 in a murine model of metastatic breast cancer

doi: 10.1097/j.pain.0000000000002953

Figure Lengend Snippet: Expression of key cytokine and chemokines in D14 marrow.

Article Snippet: A membrane-based antibody array (Proteome Profiler Mouse Cytokine Array Kit, Panel A; R&D systems) was used to detect the expression of 40 mouse cytokine and chemokines.

Techniques: Expressing

Journal: International Journal of Molecular Sciences

Article Title: Mitigating Age-Related Ovarian Dysfunction with the Anti-Inflammatory Agent MIT-001

doi: 10.3390/ijms242015158

Figure Lengend Snippet: Representative images of cytokine array blots ( A ) Assessment of 96 cytokines using a Mouse Cytokine Array Kit. Representative images of cytokine array blots are shown. MIT-001 significantly downregulated several cytokines (indicated by colored squares), including CD30L, CD30, CRG-2, IFGBP6, ILs, KC, leptin R, L-selectin, Ltn/XCL1, MCP-5, MIP-5, MIP-α, MIP-1γ, SDF-1α, TARC, TECK, TIMP-1, TNF-α, TPO, and VCAM-1, in ovaries of old mice. The positive and negative controls confirmed the reliability of the data. Data from four repeats were used for statistical analysis. ( B ) The bar graph presents the quantification of the dot intensities in the corresponding blots depending on the samples. The results in young and MIT-001-treated old mice were significantly different ( p < 0.05) from those in old mice.

Article Snippet: A cytokine array was performed using a Mouse Cytokine Antibody Array Kit (#ab193659; Abcam, Cambridge, MA, USA).

Techniques: